FastQCFastQC Report
Tue 9 Aug 2016
G132-M8-B_CCGTCC_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M8-B_CCGTCC_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15174156
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471120.31047525806377635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC191640.12629367985936088TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28850.032.7561651
ACCCGTC30350.030.73499132
TCGTATG32350.028.83493243
CGTATGC33100.028.0489244
CTCGTAT32950.027.9089842
ATCGGAA34750.027.0315152
ACGTCTG36050.026.60593415
ACACGTC35600.026.50968613
CGTCTGA37200.025.66516916
CACCCGT37100.025.4403831
TCACCCG38950.024.2882130
CCCGTCC40450.023.16921433
CACACGT43350.022.37931612
CACGTCT43350.021.92259614
TCTCGTA41550.021.76204141
CGTCCAT41300.021.73396335
CGGAAGA44350.021.5770934
CCGTCCA44800.021.11595334
GCACACG47800.020.24986511
ATCTCGT47250.018.94990740
Produced by FastQC (version 0.11.2)