FastQCFastQC Report
Tue 9 Aug 2016
G132-M6-B_GATCAG_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M6-B_GATCAG_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13423915
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17150.029.24714311
CGTCGTG18550.027.39559712
AGCGTCG20400.024.58767110
TCGTGTA21050.024.03646914
CGTGTAG21050.023.20033315
GATCTCG21950.022.7509334
GTCGTGT23950.021.31041713
GATCGGA23850.021.3073711
TCTCGGT25600.020.36649336
GTAGATC24900.020.14351531
GTCGCCG25250.020.13579844
GGTCGCC26950.020.00870943
ATCTCGG25750.019.22264535
AGAGCGT27700.018.9815148
GTAGGGA26100.018.8805818
GAGCGTC27550.018.8453039
TGGTCGC30600.017.90802242
GTGGTCG30300.016.92145341
GTGTAGG30500.016.8054216
TGTAGGG30950.016.4917
Produced by FastQC (version 0.11.2)