FastQCFastQC Report
Tue 9 Aug 2016
G132-M6-B_GATCAG_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M6-B_GATCAG_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13423915
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263300.19614248153388936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19000.027.5951821
ATCGGAA21650.024.3866062
TCGTATG22900.022.96050643
ACGATCA23650.022.3258132
ACACGTC24700.021.90968713
ACGTCTG24500.021.63958715
CTCGTAT23450.021.38993342
CGTATGC24500.021.19165844
CGATCAG24900.020.85154233
CGTCTGA26150.020.61068316
AGTCACG26450.020.37774828
GTCACGA27950.019.04829
TCACGAT28100.018.86880130
CACACGT30750.017.74208312
TCTCGTA26800.017.7312641
CACGTCT30400.017.72925614
CACGATC29950.017.63002231
CGGAAGA31800.017.0870784
GCACACG34650.015.99909111
GATCTCG31050.015.30399139
Produced by FastQC (version 0.11.2)