FastQCFastQC Report
Tue 9 Aug 2016
G132-M5-B_ACTTGA_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M5-B_ACTTGA_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16606667
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG243150.1464170986267142Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32650.035.6438711
CGTCGTG35000.033.18778612
AGCGTCG39550.029.25847410
CGTGTAG40050.029.16644315
TCGTGTA40050.029.11169214
GATCGGA39750.029.1110861
GTCGTGT41250.028.79923213
ATCTCGG42050.027.83253935
GATCTCG42200.027.57721534
TCTCGGT44950.026.33053836
GTAGATC45100.026.2912731
GTAGGGA44550.026.02376218
GTCGCCG44500.025.81779144
AGAGCGT47100.025.0822098
GAGCGTC47250.024.7232259
GGTCGCC48200.024.3832243
GTGTAGG49800.024.20709816
ATCGGAA48700.023.444892
TGTAGGG52250.022.90361817
TGGTCGC52650.022.61250342
Produced by FastQC (version 0.11.2)