FastQCFastQC Report
Tue 9 Aug 2016
G132-M5-B_ACTTGA_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M5-B_ACTTGA_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16606667
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454380.2736130013325371No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC262490.15806302372414646TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37150.032.9115681
ATCGGAA39700.030.64312
TCGTATG40550.029.83972443
CGTATGC41200.029.15544744
CTCGTAT41150.028.44221742
AATCTCG39500.028.4043139
ACACGTC44300.027.95778713
ACGTCTG45550.027.2871515
CGTCTGA46100.026.86615816
CACGTCT49850.024.80100314
TCTCGTA46000.024.77415341
CACACGT52650.023.69096812
CGGAAGA54500.022.8867784
GCACACG57100.021.6905411
ATCTCGT54500.020.78843940
TCGGAAG63350.019.550593
TTGAATC59900.019.17225336
GAATCTC71150.016.29467238
GAACTCC79750.015.9991921
CACTTGA79250.015.62888933
Produced by FastQC (version 0.11.2)