FastQCFastQC Report
Tue 9 Aug 2016
G132-M3-B_GCCAAT_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M3-B_GCCAAT_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12518697
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG134870.10773485451401213Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG23600.032.0666912
GCGTCGT23950.031.138811
TCGTGTA25700.028.84610214
CGTGTAG25650.028.7306915
GATCGGA25850.028.5946451
GTCGTGT26450.028.19551313
GATCTCG27100.026.951134
TCTCGGT28000.026.47765736
GTAGGGA28900.025.95747818
ATCTCGG29250.025.64697835
AGCGTCG29300.025.30298210
AGAGCGT30400.025.1834418
GTAGATC30100.024.48371731
TGTAGGG31700.024.35771217
GGTCGCC30600.023.87980843
GTCGCCG30950.023.46800444
GAGCGTC32200.023.4340679
AAGAGCG35000.021.6222847
GTGTAGG36400.021.51479716
TGGTCGC34850.021.47090742
Produced by FastQC (version 0.11.2)