FastQCFastQC Report
Tue 9 Aug 2016
G132-M2-B_CGATGT_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M2-B_CGATGT_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17957441
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG287930.1603402177403785Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT42350.035.32345611
CGTCGTG45000.033.29218312
AGCGTCG49200.030.62919610
GATCGGA47950.030.326211
GTCGTGT50100.030.2542813
CGTGTAG48800.029.7972415
TCGTGTA50200.029.09770614
GATCTCG50750.028.56618334
GTAGGGA50500.028.35910218
GAGCGTC55250.027.9123139
GTAGATC52550.027.83826331
TCTCGGT53400.027.56054136
AGAGCGT55500.027.2316448
ATCTCGG53600.027.08831635
GGTCGCC53750.026.86179743
GTCGCCG52750.026.66268244
TGTAGGG56050.026.25698917
TGGTCGC58150.025.01690742
GTGGTCG58450.024.88656441
ATCGGAA58100.024.8389362
Produced by FastQC (version 0.11.2)