FastQCFastQC Report
Tue 9 Aug 2016
G132-M15-B_ACTGAT_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M15-B_ACTGAT_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13961466
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG463400.33191356838887837Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT62300.038.34868611
CGTCGTG64600.036.9152212
CGTGTAG64600.036.60741415
GATCGGA65650.035.8886571
TCGTGTA66600.035.5744114
GTCGTGT67950.035.45114513
GATCTCG67450.034.5072934
TCTCGGT68700.034.29571536
AGCGTCG69100.034.28845210
ATCTCGG68350.034.0207335
GTAGGGA68650.033.80784618
AGAGCGT71500.033.5067338
GTAGATC70350.033.39692731
GAGCGTC72600.032.8778469
GTCGCCG70250.032.67844444
ATCGGAA72600.032.3318252
TGTAGGG73900.032.29820617
GTGTAGG73950.032.2763716
GGTCGCC72400.031.91899743
GTGGTCG73500.031.46752241
Produced by FastQC (version 0.11.2)