FastQCFastQC Report
Tue 9 Aug 2016
G132-M13-B_CGTACG_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M13-B_CGTACG_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14354736
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG438870.30573185044991424Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT63350.037.1227711
CGTCGTG65250.036.1092312
GATCGGA64800.035.986541
TCGTGTA67550.034.5207914
GATCTCG67450.034.0510834
AGCGTCG69300.034.03081510
GTCGTGT69950.033.9031713
CGTGTAG68850.033.77277415
GTAGGGA68150.033.7013218
ATCTCGG68550.033.4083935
TCTCGGT69900.033.1723136
GTAGATC70900.032.8279831
AGAGCGT73250.032.3759048
GTCGCCG70950.031.795744
GAGCGTC74600.031.6130759
TGTAGGG75200.031.33049817
GTGTAGG75950.031.13696916
GTGGTCG73450.030.85759741
TAGATCT75800.030.61932
GGTCGCC74000.030.48424343
Produced by FastQC (version 0.11.2)