FastQCFastQC Report
Tue 9 Aug 2016
G132-M13-B_CGTACG_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M13-B_CGTACG_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14354736
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT473040.32953584099352295TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418990.2918827625948676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA64100.038.3230821
CCGTACG65100.037.10536633
ACCGTAC66250.036.5283232
CGTACGT66500.036.324234
TACGTAA66700.035.88657836
GTACGTA68200.035.4198735
CGTAATC62950.034.91246838
ACGTAAT65600.034.81014637
CACCGTA70900.034.1648231
ATCGGAA71900.034.0536352
ACGTCTG76750.032.1596815
AATCTCG68450.032.10801341
ACACGTC76550.032.04253413
CTCGTAT72750.031.84298544
CGTCTGA78450.031.49082416
GTCACCG79200.030.88890529
CACGTCT82950.029.83549914
TCTCGTA74700.029.65679743
CGGAAGA82350.029.6254634
TCACCGT84150.029.20364630
Produced by FastQC (version 0.11.2)