FastQCFastQC Report
Tue 9 Aug 2016
G132-M11-B_GTGGCC_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M11-B_GTGGCC_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13519742
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG230920.1708020759567749Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT33800.035.14705311
CGTCGTG35500.033.52580612
CGTGTAG35550.032.7963615
TCGTGTA36800.031.9814814
GATCGGA36950.030.8997631
GTCGTGT39300.030.61987513
GATCTCG38850.029.89886934
AGCGTCG39800.029.84850510
ATCTCGG39100.029.65143835
AGAGCGT41800.028.7887578
GTAGGGA41450.028.3417718
GTAGATC40850.028.21888431
TCTCGGT41400.028.16355136
GAGCGTC44250.027.0456569
TGGTCGC42250.027.0332842
GTCGCCG42000.026.88269444
TGTAGGG45650.026.40761417
GTGGTCG43450.026.23524941
GGTCGCC44000.025.95963143
GTGTAGG46350.025.81894716
Produced by FastQC (version 0.11.2)