FastQCFastQC Report
Tue 9 Aug 2016
G132-M11-B_GTGGCC_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M11-B_GTGGCC_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13519742
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254980.18859827354693603No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT252890.1870523860588464TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA35450.034.302491
ATCGGAA38000.031.9562322
TATCTCG36050.031.61199241
ACACGTC39550.030.81508413
CTCGTAT38750.030.77160644
ACGTCTG42100.029.15762715
AGTCACG41950.028.7388628
CGTCTGA43100.028.2769516
GTCACGT42900.028.20501529
ACGTGGC43150.027.99020632
CACGTCT44350.027.72798314
CACACGT45900.026.55199612
TCTCGTA44950.025.64626943
TCACGTG48000.025.43777530
GCCTTAT46300.025.42059537
CGGAAGA48800.025.064274
CGTGGCC49150.024.573233
GCACACG51300.023.628411
GGCCTTA52950.022.85157236
ATCTCGT52050.022.10566142
Produced by FastQC (version 0.11.2)