FastQCFastQC Report
Tue 9 Aug 2016
G132-M10-B_GTGAAA_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M10-B_GTGAAA_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12760577
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG641930.50305718934183Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT419880.32904468191367836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA331000.25939265912505366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT79150.040.74640311
CGTCGTG80550.039.9835912
GTCGCCG80350.038.70770644
GATCTCG80800.038.60714734
GATCGGA81100.038.5187341
CGTGTAG82950.038.4013515
GTCGTGT86050.037.862613
GTAGGGA83500.037.5963318
TCGTGTA85050.037.50489814
ATCTCGG83400.037.42994335
TCTCGGT84950.037.29082536
AGCGTCG86400.037.27636710
AGAGCGT87250.036.8375748
GAGCGTC87050.036.7453089
GTAGATC86800.036.29284731
ATCGGAA86450.035.9314082
GTGTAGG91000.035.51196716
GTGGTCG88300.035.28945541
TAGATCT90050.035.10542332
GGTCGCC89600.035.1027843
Produced by FastQC (version 0.11.2)