FastQCFastQC Report
Fri 22 Apr 2016
G130-M8_AGTTCC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M8_AGTTCC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5375007
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT718771.3372447700998344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5850.023.7133081
CCGTATC5850.021.43653738
TCCGTAT6250.020.41660737
GTTCCGT6900.019.13100435
ACGTCTG7250.018.8131315
TATCTCG6850.018.62811941
ACACGTC7250.018.50969113
AGTTCCG8150.018.08541934
CTCGTAT7700.017.71514344
TTCCGTA8100.017.11162236
ATCGGAA8200.016.6335582
CGTCTGA8250.016.26609216
TCTCGTA8900.015.82054943
ACGCGGT1552.673296E-715.61235128
CGTATCT8050.015.57810339
CACACGT9450.014.66614912
CGGAAGA11500.014.1560234
CACGTCT10550.013.55402414
GGCGTAT3800.012.7481321
GTCGGTT7100.012.7154871
Produced by FastQC (version 0.11.2)