FastQCFastQC Report
Fri 22 Apr 2016
G130-M7_AGTCAA_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M7_AGTCAA_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12384150
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214760.1734152121865449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG16100.026.91855612
GCGTCGT16750.026.13600211
CGTGTAG18450.023.132115
GATCGGA19250.021.8310891
TCGTGTA19600.021.77485714
AGCGTCG21650.020.72883610
GTCGTGT21700.020.37726813
TCTCGGT21950.020.0452836
GATCTCG21700.019.97223534
GTCGCCG21700.019.3632444
GTAGATC23150.019.1971331
AGAGCGT23300.019.1666538
GAGCGTC22950.018.979679
GTAGGGA23350.018.56197218
GTGGTCG23500.018.06725141
GTGTAGG25250.017.25096716
ATCTCGG25650.016.9822935
GGTCGCC26700.016.4786443
TGGTCGC27150.015.88140242
TGTAGGG27600.015.86312417
Produced by FastQC (version 0.11.2)