FastQCFastQC Report
Fri 22 Apr 2016
G130-M4_GATCAG_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M4_GATCAG_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689483
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT776090.6116009612054328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310320.24454897019839184No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG155080.12221144076555365Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25050.031.7077711
GCGTCGT25300.031.47720111
CGTCGTG25600.030.76495412
TCGTGTA28650.027.5665914
GTCGTGT29550.027.0992413
AGAGCGT29550.027.0991338
TCTCGGT28700.026.98241436
CGTGTAG29400.026.56404515
GTCGCCG29000.026.39900844
GTGGTCG29150.026.18738441
ATCGGAA30900.025.7040332
AGCGTCG32000.024.81801210
GAGCGTC31850.024.7967519
GGTCGCC30900.024.77547543
TGGTCGC32650.023.64967242
GTGTAGG34100.023.48335816
GTAGATC32500.023.28674731
CTCGGTG33300.022.92433437
GATCTCG33350.022.62672634
GTAGGGA34600.022.44583518
Produced by FastQC (version 0.11.2)