FastQCFastQC Report
Fri 22 Apr 2016
G130-M4_GATCAG_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M4_GATCAG_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689483
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586310.4620440407225417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336740.2653693613837538No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC166330.13107705018399884TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22700.035.7927251
ACACGTC26000.031.64490913
TCGTATG25700.031.5886743
ACGTCTG26450.031.02335715
CTCGTAT25950.030.94497942
CGTCTGA26450.030.85701216
ACGATCA26900.030.09558932
CGTATGC27200.029.6048144
CGATCAG27400.029.54640233
ATCGGAA27800.029.2796552
TCACGAT29700.027.33236530
GTCACGA30100.026.9690429
CACGTCT30950.026.65484814
CACGATC32100.025.5629631
CACACGT32500.025.38361712
TCTCGTA32200.024.46001641
AGTCACG34250.023.76548828
GCGCTGT22750.023.01602242
CGGAAGA36500.022.3004914
GGGCGCT23000.022.28783640
Produced by FastQC (version 0.11.2)