FastQCFastQC Report
Fri 22 Apr 2016
G130-M3_ACTTGA_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M3_ACTTGA_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12794606
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT17750.030.7366811
CGTCGTG20200.026.9002312
AGCGTCG22600.024.23787510
GATCGGA23000.024.010741
TCGTGTA23100.023.90408714
GTCGTGT24600.022.62537813
CGTGTAG24200.022.54481915
GATCTCG24150.022.13705834
TCTCGGT24600.021.8211236
GTAGATC24550.021.59781831
GAGCGTC26800.020.2754679
GTCGCCG26500.020.25571444
ATCTCGG27300.020.22732535
ATCGGAA26950.020.1647432
AGAGCGT28150.019.537568
GTAGGGA27500.019.44051618
GGTCGCC28300.018.81167643
GTGGTCG28900.018.80179641
TGTAGGG31900.018.00047717
TGGTCGC30450.017.70016542
Produced by FastQC (version 0.11.2)