FastQCFastQC Report
Fri 22 Apr 2016
G130-M2_CAGATC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M2_CAGATC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324789
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG175080.142055170275126Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT27100.033.28269611
CGTCGTG28600.031.53709812
TCTCGGT31550.028.30962436
TCGTGTA31900.028.13671314
CGTGTAG31750.027.923215
GATCGGA32100.027.76021
GATCTCG31650.027.66467534
GTCGTGT32700.027.51562913
GTCGCCG31650.027.45536844
AGCGTCG33850.026.90577710
GTAGATC33350.026.7178731
GTAGGGA33700.026.30992118
AGAGCGT35200.026.0611618
GAGCGTC35850.025.0977349
ATCGGAA35350.025.0828932
GGTCGCC35350.024.64381243
ATCTCGG36250.024.33589435
TGTAGGG38000.023.85382717
GTGTAGG38850.023.49962616
GTGGTCG37200.023.41824741
Produced by FastQC (version 0.11.2)