FastQCFastQC Report
Fri 22 Apr 2016
G130-M1_GCCAAT_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M1_GCCAAT_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12662659
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA20250.031.6387421
ATCGGAA23500.027.6160962
CGTATGC23650.026.88589944
ACACGTC24450.026.63284313
ACGCCAA23850.026.56504432
TCGTATG24450.026.36520843
ACGTCTG24800.026.25697915
GTCACGC24350.026.01945729
CGCCAAT24350.025.8388733
CGTCTGA27000.024.28047616
AGTCACG27250.023.41187528
CTCGTAT27900.022.78947642
TATCTCG28700.022.307939
TCACGCC30200.021.27072730
CACGTCT31700.020.95812414
CACGCCA31050.020.68843731
CACACGT32350.020.26500312
TCTCGTA31300.020.24349241
CGGAAGA33150.020.1077654
TCGGAAG37950.018.144323
Produced by FastQC (version 0.11.2)