FastQCFastQC Report
Mon 25 Apr 2016
G129-M8_ACTTGA_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M8_ACTTGA_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19494518
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15899708.155985185168467Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1874600.043.4987341
GCGTCGT1907500.043.47179411
TCGTGTA1883950.043.4324314
CGTCGTG1908650.043.41667612
AGAGCGT1903950.043.3827178
GTCGTGT1925150.043.35765513
ATCGGAA1871000.043.3378452
GTAGGGA1851200.043.3304318
CGTGTAG1890650.043.2910915
AGCGTCG1899050.043.2845610
GATCTCG1882500.043.26927634
TCTCGGT1899300.043.26491536
TGTAGGG1919450.043.2486917
AAGAGCG1877450.043.235557
GTAGATC1894300.043.23085431
ATCTCGG1889050.043.2112535
GAGCGTC1899450.043.1972969
GTGGTCG1876900.043.09983441
GTGTAGG1925900.043.08638416
TGGTCGC1852400.043.0549642
Produced by FastQC (version 0.11.2)