FastQCFastQC Report
Mon 25 Apr 2016
G129-M7_CAGATC_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M7_CAGATC_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15547911
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4422912.8446972715498564TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA519800.043.341271
ATCGGAA525100.042.7697532
CGTATGC522550.042.58760544
ACACGTC531000.042.3399713
CGTCTGA532600.042.24581516
TCGTATG528850.042.16602743
ACGTCTG535150.042.10617415
CTCGTAT517500.042.09561542
CACACGT536150.041.98250612
CACGTCT540050.041.7526514
TCTCGTA499100.041.5925541
GCACACG544450.041.28996711
ATCTCGT502600.041.25024440
CGGAAGA542000.041.188444
TCGGAAG544850.041.1588483
ACCAGAT550450.040.0247532
AGTCACC558050.039.91113728
GATCATC514550.039.82531436
CATCTCG520400.039.70772639
GAACTCC565900.039.67048321
Produced by FastQC (version 0.11.2)