FastQCFastQC Report
Mon 25 Apr 2016
G129-M12_CCGTCC_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M12_CCGTCC_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18086219
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2797621.5468241316772733Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT359100.042.59431511
CGTCGTG361000.042.31505212
TCGTGTA357700.042.15205414
GATCGGA359450.041.899961
CGTGTAG361700.041.7646215
GTAGATC360450.041.759331
GTCGTGT369600.041.66971213
AGCGTCG363350.041.6607610
TCTCGGT363300.041.551936
GATCTCG360650.041.41882734
GTAGGGA356650.041.3140418
TGTAGGG373150.041.06717317
ATCGGAA363900.041.0384182
ATCTCGG364950.041.0152135
GAGCGTC369050.040.956089
GTGTAGG375500.040.78650316
AGAGCGT372700.040.774338
GTGGTCG365000.040.73701541
TGGTCGC360300.040.73212442
AAGAGCG368400.040.48577
Produced by FastQC (version 0.11.2)