FastQCFastQC Report
Mon 25 Apr 2016
G129-M12_CCGTCC_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M12_CCGTCC_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18086219
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC3004311.661104512778486TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA373750.042.689671
CGTATGC373500.041.96592344
ATCGGAA378950.041.8961682
ACCCGTC371450.041.67595332
TCGTATG377050.041.66291443
CTCGTAT368850.041.28381742
ACGTCTG389150.041.13687515
ACACGTC389450.041.12213513
CGTCTGA388900.041.0445316
CACCCGT378600.041.00098831
TCACCCG381300.040.8029730
TCTCGTA355700.040.76207741
CACACGT394600.040.59101512
CACGTCT396400.040.4732914
CCCGTCC382650.040.30088833
ATCTCGT360300.040.27230540
CGTCCAT368450.039.77044335
TCGGAAG402550.039.5164643
GCACACG405000.039.45090511
CCGTCCA389900.039.43313634
Produced by FastQC (version 0.11.2)