FastQCFastQC Report
Mon 25 Apr 2016
G129-M11_GGCTAC_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M11_GGCTAC_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19890126
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3379071.6988680715245346Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT430250.042.9032611
CGTCGTG434900.042.38372812
TCGTGTA430900.042.1595414
CGTGTAG432250.042.0681615
GATCGGA430850.042.012631
AGCGTCG434550.041.9627810
GATCTCG432300.041.8723434
GTAGATC436550.041.83268431
TCTCGGT439850.041.73306736
GTCGTGT447350.041.57298713
GTAGGGA426800.041.46109418
ATCTCGG438200.041.44443535
TGTAGGG445650.041.44001417
AGAGCGT440000.041.433438
ATCGGAA432900.041.3678252
GAGCGTC440550.041.3109449
GTGTAGG449500.041.0702916
GTGGTCG437100.041.05331841
TGGTCGC428950.041.03953642
AAGAGCG438850.040.734737
Produced by FastQC (version 0.11.2)