FastQCFastQC Report
Sun 24 Apr 2016
G130-M9_GGCTAC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M9_GGCTAC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208916
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA6450.011.59639227
AATTGCG6800.011.32408836
CTCGAAT15200.09.9943351
CTCGTAT6000.09.9074281
CTCGGTT14300.09.699581
TCGGAAT7550.09.6155672
TGCGATA7850.09.5291839
GGCGAAT5651.8189894E-129.3521441
CCGGGAT10900.09.2913691
CGATTGG9250.09.27526954
TCGGTTT18150.09.09062
CGAATGT13100.09.0683953
GCCGATT4652.8230716E-98.9959921
GTCGGTT17450.08.9580051
AACGTGC10250.08.79961419
CAACGAT10050.08.7627891
CGGGATT7300.08.7462841
TCGGGAT7300.08.7394292
ATTACGG7550.08.450788539
CGGGCTA4451.2010969E-78.41081
Produced by FastQC (version 0.11.2)