FastQCFastQC Report
Sun 24 Apr 2016
G130-M16_CGTACG_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M16_CGTACG_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528480
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC8200.012.0735053
CCACTAG29250.010.454993
TAGCTCG15700.09.38887122
CGTATTA8950.09.34022110
CCGTTAA8500.09.31775718
CGACGAT6101.8189894E-129.0172911
GGAAAAC70700.08.5581371
AATCGGC4703.263449E-88.4257665
CCCCGTA10050.08.3183747
CCGGAAT18500.08.2062231
CGAATAA8600.08.18620920
CACTAGC37400.08.1766984
CGATAGA3004.0340397E-48.0668914
CTACGCA14050.07.9857328
ACGCTAA6901.6370905E-117.971267726
CACGATA13350.07.909574542
CACGATC13950.07.886091
GGGGAAT31600.07.8678721
CCCACTA39000.07.8417752
ACGATCA13200.07.8340822
Produced by FastQC (version 0.11.2)