FastQCFastQC Report
Sun 24 Apr 2016
G130-M15_GTTTCG_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M15_GTTTCG_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544482
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC11500.014.9216443
CACGATC15800.012.1147861
ACGATCA17050.010.9684012
CCGGAAT20250.09.9957731
CGATACA15000.09.67958144
GGGGGAT20300.09.3208611
CACGATA15400.08.85669442
CGACAAT7600.08.6848291
ACGATAC16900.08.59128143
TCACGAT17650.08.47552341
GGAAAAC71700.08.3464781
CGGAATT19800.08.1115982
ACGCAAC9300.08.04266310
CTAACGC7501.8189894E-127.92038823
GGGAAAT43650.07.91350751
GGAAAAT68400.07.88067771
AATCGAT9850.07.81720925
CCCCGTA9300.07.80627
TAGCTCG12450.07.775481722
ACGCTAA7103.092282E-117.746743726
Produced by FastQC (version 0.11.2)