FastQCFastQC Report
Sun 24 Apr 2016
G130-M15_GTTTCG_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M15_GTTTCG_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544482
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT8450.010.6833991
TCGGTTT22400.010.41037752
CTCGAAT22800.010.3331151
CGGGCGT5350.010.288891
CGCGATA4153.601599E-1010.0806341
AATCCGA15800.09.88568324
GTCGGTT19950.09.8226671
CGAATGT18200.09.7908923
CGGGCTA6450.09.2169311
CGGGCTT25150.09.1924991
GGCGAAT5803.6379788E-129.110991
CTCGTTT25150.09.1049521
GTCGCTT10400.09.103691
ACCGGTA12200.08.83571427
CGGGTTT17250.08.807291
ATCCGAA16750.08.7996525
AATTGCG12250.08.62070236
CGTATAG4856.0990715E-98.61821419
CGGTTTG23750.08.521833
AAATCCG17600.08.49966123
Produced by FastQC (version 0.11.2)