FastQCFastQC Report
Sun 24 Apr 2016
G130-M14_GTGGCC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M14_GTGGCC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564798
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG24200.012.7274553
CGACGAT3650.012.6587081
ATCGCTC9750.010.83018742
ACGCAAC5250.010.05676110
CCCACTA31250.09.9272172
CCCCACT39750.09.7971341
CGATACA11150.09.66779344
CTTAGAT18850.09.4544571
ATCTACG10950.09.4426626
TACGCAA5751.8189894E-129.1823079
CACTAGC33150.09.0921354
CCGGAAT15100.09.033951
CACGATA11950.09.02043642
TTGCGCG2701.2917424E-48.9626228
TAGATCC16050.08.9097843
CGCAACG5456.91216E-118.88072132
ACTAGCC34500.08.8638945
CGAATAA8750.08.80040220
GGAAGAT61600.08.7508121
TTAGATC17650.08.7259292
Produced by FastQC (version 0.11.2)