FastQCFastQC Report
Sun 24 Apr 2016
G130-M13_GTGAAA_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M13_GTGAAA_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10364255
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10150.013.4395581
CGGGGAT13100.010.5810461
CCGGAAT14100.010.2987411
CCACTAG23150.010.0733133
CGAATAA8300.09.27722120
CGACGAT5253.092282E-119.2198271
TACGCAA6057.2759576E-128.7270839
GGAAGAT69150.08.6861991
CGGACAT12200.08.6564681
GTCGATT3055.0914343E-58.6564681
ACGCAAC6109.094947E-128.65542410
GCATATC19100.08.52341656
CGGGAAT11100.08.5232421
CGACACT8050.08.4727651
GGAAAAC59550.08.3130451
GGGGAAT23300.08.3097141
CACGATA11650.08.30871242
AATCGAA6901.8189894E-128.2897155
GGGAAAT34050.08.2708891
CGGGTAT4804.6589776E-88.2506971
Produced by FastQC (version 0.11.2)