FastQCFastQC Report
Sun 24 Apr 2016
G130-M13_GTGAAA_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M13_GTGAAA_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10364255
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG1802.8922502E-410.9995839
CTCGAAT16050.010.9755351
TCGGTTT18600.010.4083072
AATCCGA12150.010.32059624
CGCTATT3654.7657522E-910.2557361
AATTGCG9450.010.2436436
ACCGGTA9150.010.09797827
GTCGGTT16600.010.0812941
CCGGAAT7250.010.0227451
ATCCGAA12450.09.71850725
CGCCTAT3452.371562E-79.5737691
GTCGGAT6700.09.5309011
CGCGTAT1900.0055232219.2714391
CGAATGT14600.09.1915213
TCGGATC16350.09.1495892
AAATCCG13300.09.097423
TAAGGCG15050.09.0627789
CTCGGAT22450.09.023651
TGCGATA10350.08.927839
ATTGCGA10400.08.88483537
Produced by FastQC (version 0.11.2)