FastQCFastQC Report
Sun 24 Apr 2016
G129-M15_AGTTCC_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M15_AGTTCC_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698340
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC21500.022.6132661
ACGATCA22800.020.9392812
CGATCAC23200.020.1085413
GCGTCGT8850.014.41696111
CGTCGTG10800.011.81375912
AGCGTCG12100.011.63555210
TCGTGTA10550.011.05123114
CGTGTAG11600.010.99906115
CCGGAAT22400.010.7050321
GATCTCG11100.010.70250434
TAATGTA45550.010.3868244
AATGTAG51950.09.9543975
TCACAAG52450.09.8175486
CGACGAT6350.09.7004931
CGATACA16000.09.21276544
TAATGGG49500.09.02177119
ATCTCGG13650.08.86430535
ATTAACG21900.08.83977620
TCTCGGT14200.08.8304436
CACGATA16700.08.82618442
Produced by FastQC (version 0.11.2)