FastQCFastQC Report
Sun 24 Apr 2016
G129-M13_CTTGTA_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M13_CTTGTA_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304942
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC30350.023.9201011
ACGATCA32900.021.933312
CGATCAC35400.020.6375893
AATGTAG71200.011.249825
TAATGTA67750.011.205574
TCACAAG70300.010.986926
ATGTAGA83000.09.8890576
CTTTAAT94900.09.7594131
TTAATGT86250.09.6950183
TAATGGG72000.09.59392619
GTAGAAA76100.09.4266438
CGAATAA13100.09.06836120
GGCAACT85500.08.9798091
CCGGAAT25400.08.9209541
GATCACA92100.08.8163914
TTAACGA29950.08.8143621
TTTAATG99600.08.7470592
ACTTTAG79350.08.7077695
TTAGACT74300.08.6776668
ATTAACG29950.08.66745420
Produced by FastQC (version 0.11.2)