FastQCFastQC Report
Tue 17 May 2016
G131-M8-B_GTGGCC_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M8-B_GTGGCC_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412536
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG445200.255677863350864Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261180.14999538263696913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT65100.037.57774411
CGTCGTG68300.035.4950512
TCGTGTA68150.035.08896614
GATCTCG67950.034.73898734
GTCGTGT71750.034.1566413
TCTCGGT70000.033.8473436
GATCGGA70700.033.823381
AGCGTCG71850.033.8025310
CGTGTAG71150.033.4857815
AGAGCGT75250.032.7137578
GTAGATC73650.032.3778831
GAGCGTC75550.032.2053159
ATCGGAA73700.032.1182332
ATCTCGG73400.032.0996535
GTAGGGA72400.031.57103518
GTCGCCG74200.030.96754844
TGGTCGC75500.030.72187242
TGTAGGG79450.030.34754217
GTGTAGG79900.030.23169116
GTGGTCG77450.030.0317241
Produced by FastQC (version 0.11.2)