FastQCFastQC Report
Tue 17 May 2016
G131-M8-B_GTGGCC_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M8-B_GTGGCC_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412536
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT501960.2882750680314458TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65800.038.8087161
ATCGGAA70050.036.272012
GTCACGT72750.034.74629629
ACGTCTG73600.034.43280815
ACACGTC73600.034.2833613
CGTCTGA75800.033.8397516
CTCGTAT72000.033.82500544
AGTCACG74800.033.79402528
ACGTGGC76050.033.4121432
TATCTCG70000.032.71761741
CACACGT80150.031.53655812
TCACGTG80500.031.37382930
CACGTCT81450.031.24928314
CGTGGCC83950.030.24146833
CGGAAGA84700.030.2320354
TCTCGTA77450.029.8825143
TCGGAAG90050.028.6557713
GCACACG91350.027.86266111
CACGTGG92100.027.6850531
GCCTTAT84750.027.59370637
Produced by FastQC (version 0.11.2)