FastQCFastQC Report
Tue 17 May 2016
G131-M6-B_GTCCGC_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M6-B_GTCCGC_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368494
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG454560.39984187879238886Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT62650.040.3466111
CGTCGTG65150.038.73085812
TCGTGTA65900.037.9226914
AGCGTCG67350.037.3025510
GATCGGA66400.037.1073571
GTCGTGT69300.036.95130513
GATCTCG67900.036.8379134
TCTCGGT70000.036.04704736
CGTGTAG69150.036.0449115
GTAGATC69700.035.6328231
GAGCGTC70950.035.5028429
ATCTCGG70750.035.41617235
GTAGGGA68650.035.41047718
ATCGGAA69350.035.306832
GTCGCCG67400.035.29998844
AGAGCGT72800.035.0841458
TGTAGGG73300.034.78449617
TGGTCGC69250.034.4798442
GTGTAGG75550.033.83591516
GTGGTCG72800.033.2799441
Produced by FastQC (version 0.11.2)