FastQCFastQC Report
Tue 17 May 2016
G131-M5-B_CCGTCC_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M5-B_CCGTCC_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190693
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC318300.31234382195597493TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45050.038.2872471
CGTATGC44850.037.27995744
TCGTATG45050.037.26095643
ACCCGTC45400.036.44101732
ATCGGAA47750.035.520552
ACACGTC48400.034.95261413
ACGTCTG49450.033.81006215
CTCGTAT48550.033.80444342
CACCCGT50350.033.07674431
CGTCTGA51000.033.0413116
TCACCCG51550.032.4773230
TCTCGTA50050.031.38503541
CACACGT54200.031.2934712
CACGTCT54100.031.10733614
CCCGTCC53950.030.6658433
CGGAAGA55300.030.5914364
TCGGAAG59900.028.829793
GCACACG59550.028.70370311
CCGTCCA59050.028.0914134
ATCTCGT55550.027.8815640
Produced by FastQC (version 0.11.2)