FastQCFastQC Report
Tue 17 May 2016
G131-M14-B_AGTCAA_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M14-B_AGTCAA_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9412567
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479400.5093190837313561No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG424860.4513752730790655Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT59300.039.9550811
CGTCGTG60700.038.88857312
GATCGGA59550.038.8264161
TCGTGTA61200.037.9587514
GTCGTGT62900.037.70327413
GATCTCG61000.037.54165334
CGTGTAG61850.037.41755715
AGCGTCG62950.037.35899710
GTAGATC62250.037.24641431
ATCGGAA62100.037.1966672
ATCTCGG61800.037.09127435
GTCGCCG61700.036.95886644
AGAGCGT64600.036.881558
TCTCGGT62300.036.828936
GTAGGGA61650.036.21885318
GAGCGTC65550.035.9107369
GGTCGCC64750.035.1826443
TGTAGGG67100.035.14565717
TGGTCGC64750.035.1120442
GTGTAGG68350.034.50290716
Produced by FastQC (version 0.11.2)