FastQCFastQC Report
Tue 17 May 2016
G131-M13-B_ATTCCT_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M13-B_ATTCCT_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13910849
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172270.12383859532944395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16200.026.7915971
TATCTCG16150.023.02182841
ATCGGAA19450.022.394672
CGTCTGA21000.020.9512416
CTCGTAT20500.019.85372444
ACACGTC23650.017.95251313
ACGTCTG24650.017.13497415
TCTCGTA23050.016.79831743
CACACGT27150.015.80024712
CGGAAGA31600.015.0371584
ATCTCGT26500.014.27929142
CACGTCT30100.014.10554714
GCACACG33550.012.58947911
TCGGAAG39650.011.9287343
AATCCGA16000.011.27438724
CGGGCTA6650.010.9330081
GGTACGA11400.010.806434
ATCCGAA17450.010.58994925
TACGAGG11850.010.5817036
AAATCCG17500.010.55942623
Produced by FastQC (version 0.11.2)