FastQCFastQC Report
Tue 17 May 2016
G131-M12-B_ACTGAT_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M12-B_ACTGAT_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182051
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG377950.24894528413848693Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT51550.037.5971611
CGTCGTG54750.035.1989112
TCGTGTA56600.034.1645714
GATCTCG56800.033.57961734
TCTCGGT57450.033.5060336
CGTGTAG57700.033.28449215
AGCGTCG58800.033.11111510
GATCGGA58550.032.463561
GTAGATC60450.032.0599731
GTCGTGT61900.031.77284813
GTCGCCG59000.031.70855744
ATCTCGG60300.031.52110335
GTGGTCG61250.030.93443941
TGGTCGC61600.030.61789742
GTAGGGA62100.030.60744518
GAGCGTC64050.030.2940449
GGTCGCC63200.029.56593343
AGAGCGT67700.029.4081548
TGTAGGG67450.029.38639817
GTGTAGG67650.029.2995216
Produced by FastQC (version 0.11.2)