FastQCFastQC Report
Tue 17 May 2016
G131-M12-B_ACTGAT_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M12-B_ACTGAT_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182051
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT410980.27070123792892015TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA55700.036.7105061
TATCTCG53750.035.2408741
CTCGTAT57700.034.2386644
ATCGGAA60350.033.8638272
ACACGTC62050.033.1133213
CGTCTGA62350.032.7775816
TCTCGTA59250.032.30331843
ACGTCTG64450.031.81197215
ATCTCGT63850.029.80378542
CACGTCT69650.029.72118614
CACACGT70350.029.45672212
ATATATC67900.027.99359938
GATATAT69300.027.93618437
CGGAAGA75250.027.8017184
TCGGAAG78200.026.6404083
GCACACG78600.026.22494911
TGATATA77600.026.22392336
ATATCTC74000.025.8053640
CTGATAT83350.024.52041235
ACTGATA83000.024.5178734
Produced by FastQC (version 0.11.2)