FastQCFastQC Report
Tue 17 May 2016
G130-M2_CAGATC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M2_CAGATC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324789
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198890.16137395942437635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC187980.15252188090197732TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA27550.033.4885181
TCGTATG30100.030.84473643
CGTATGC30450.030.20180744
ATCGGAA30800.029.8560582
ACACGTC31550.029.4251213
ACGTCTG31500.029.19247215
CTCGTAT31250.029.07591242
CGTCTGA32200.028.83113516
CACGTCT36950.025.30344414
TCTCGTA35950.024.90732641
CACACGT38050.024.45630512
CGGAAGA39800.023.602064
TCGGAAG42750.022.3336933
GCACACG45550.020.57436211
ATCTCGT45800.019.50313640
ACCAGAT51450.018.68534932
CATCTCG51400.017.84912139
AGTCACC54600.017.24472428
GAACTCC60750.016.00593821
GATCATC57300.015.97285736
Produced by FastQC (version 0.11.2)