FastQCFastQC Report
Tue 17 May 2016
G129-M1_ATCACG_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M1_ATCACG_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17441727
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8162544.679892077200842Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT993150.043.33949311
CGTCGTG993650.043.2532312
TCGTGTA980600.043.22313714
GTCGTGT1005500.043.1197913
AGCGTCG987250.043.09502810
CGTGTAG984150.043.07380715
ATCGGAA980400.043.04022
GATCGGA987900.043.0255971
GATCTCG981950.042.9702934
TCTCGGT992750.042.96079636
AGAGCGT996600.042.9395838
GTAGGGA967050.042.87838718
TGTAGGG1002600.042.85225317
GTAGATC992250.042.8260131
GTCGCCG968300.042.80565644
TGGTCGC969050.042.73687742
GAGCGTC994750.042.706189
GTGGTCG983450.042.6917541
ATCTCGG990850.042.684635
GTGTAGG1008850.042.59282316
Produced by FastQC (version 0.11.2)