FastQCFastQC Report
Tue 17 May 2016
G128-M9-B_GATCAG_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M9-B_GATCAG_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15465277
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1434810.9277622379476294Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189490.12252609507091272No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156860.10142721659624977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA195000.040.96392414
GATCGGA194950.040.9073831
CGTGTAG195050.040.84050415
GTCGTGT203650.040.4878813
GATCTCG195850.040.38149634
TCTCGGT197600.040.32446736
AGAGCGT202150.040.2224168
GCGTCGT204450.040.13590611
ATCGGAA196400.040.1125032
CGTCGTG204500.040.05065512
GTGGTCG196950.039.82089641
ATCTCGG198600.039.7669535
GTAGATC200650.039.76504531
GTAGGGA195050.039.44180718
TGTAGGG207300.039.0958117
GTGTAGG206900.038.9587416
AAGAGCG206650.038.4308747
GAGTGTA212650.038.17255427
TGGTCGC203600.038.1547642
GAGCGTC211950.038.113539
Produced by FastQC (version 0.11.2)