FastQCFastQC Report
Tue 17 May 2016
G128-M8-B_ACTTGA_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M8-B_ACTTGA_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12676090
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1219030.9616766684364028Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451170.35592205482920997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA166150.041.4836961
CGTGTAG165950.041.43942615
TCGTGTA167500.041.26609414
AGAGCGT173150.040.92318
ATCGGAA168100.040.7799952
GTCGTGT176700.040.66133513
TCTCGGT171500.040.41986536
GATCTCG169450.040.41535634
ATCTCGG169500.040.40359535
GTAGATC172100.040.03386331
CGTCGTG179050.039.7099212
AAGAGCG174600.039.6886757
GTAGGGA167850.039.62008318
GTGGTCG170150.039.61543341
GGTCGCC167200.039.58233643
GCGTCGT181250.039.31288511
GTGTAGG180100.038.977616
TGTAGGG180100.038.96538517
CTCGGTG177300.038.8618637
GAGTGTA182100.038.6453927
Produced by FastQC (version 0.11.2)