FastQCFastQC Report
Tue 17 May 2016
G128-M7-B_CAGATC_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M7-B_CAGATC_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9974799
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC6056246.071540890197387TruSeq Adapter, Index 7 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592740.594237538019563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317950.31875329016654874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA712150.043.6937681
ATCGGAA713200.043.3282932
CGTATGC710450.043.2181944
ACACGTC711550.043.05776213
CGTCTGA714600.043.00328416
ACGTCTG715050.042.99160415
TCGTATG719700.042.8919843
CACGTCT715800.042.8820214
CGGAAGA714750.042.8711474
CTCGTAT706200.042.84902242
CACACGT718450.042.74221812
TCGGAAG722000.042.6752663
TCTCGTA684650.042.56221441
CATCTCG686800.042.25557739
ATCTCGT691500.042.15649840
ACCAGAT728700.042.0149832
GAACTCC739500.041.97475421
CTGAACT742200.041.93172519
GCACACG741900.041.9071711
GATCATC686600.041.90367536
Produced by FastQC (version 0.11.2)