FastQCFastQC Report
Tue 17 May 2016
G128-M5A-B_ACAGTG_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M5A-B_ACAGTG_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11650472
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3493542.9986252917478367TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA425300.043.480191
ATCGGAA428150.042.9952132
CGTATGC428900.042.6806144
ACACGTC431400.042.36534513
TCGTATG434450.042.36324343
CTCGTAT419900.042.3589642
ACGTCTG433400.042.2713615
CGTCTGA434250.042.13795516
CACGTCT435900.041.9784514
CACACGT437300.041.93461212
ATCTCGT409900.041.5568440
CGGAAGA441300.041.5345734
TCTCGTA410700.041.524141
TCGGAAG445100.041.4073223
AGTGATC407250.041.37906636
GCACACG446450.041.3560211
GATCTCG411950.041.1891239
GAACTCC461000.039.9413421
CTGAACT465000.039.6589219
GTCTGAA463700.039.4949517
Produced by FastQC (version 0.11.2)