FastQCFastQC Report
Tue 17 May 2016
G128-M14-B_ATGTCA_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M14-B_ATGTCA_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16435422
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG337830.20554993963647541Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT57200.035.8835211
CGTCGTG59950.034.56785612
TCGTGTA60700.033.23467314
GTCGCCG58000.032.59723744
CGTGTAG61400.032.35415615
GATCTCG60150.032.1846334
AGCGTCG63800.031.86099810
GTCGTGT65950.031.7231613
TCTCGGT62300.031.70952636
GATCGGA62550.031.6186351
GTAGATC64200.030.83905231
ATCTCGG63850.030.49185635
GAGCGTC67250.030.3900579
GTAGGGA63600.029.71256618
AGAGCGT69800.029.6265138
TGGTCGC64000.029.33115242
GGTCGCC64100.029.28833643
ATCGGAA68050.028.9338112
GTGTAGG70850.028.50450716
GTGGTCG68350.028.23570841
Produced by FastQC (version 0.11.2)