FastQCFastQC Report
Tue 17 May 2016
G128-M14-B_ATGTCA_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M14-B_ATGTCA_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16435422
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT392450.2387830382450782TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177390.10793151523581203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA59700.036.2816351
CTCGTAT61050.033.512744
ATCGGAA63950.033.5051842
ACACGTC65600.033.0648613
CGTCTGA65950.032.58917616
ACGTCTG66650.032.57696515
AATCTCG63200.030.8410241
TCTCGTA66250.030.08530443
CACGTCT72750.029.81518614
CACACGT75600.029.04038412
CGGAAGA78200.027.877934
TCGGAAG81750.026.9095133
GCACACG82850.026.4725811
ATCTCGT78850.025.08245842
GAATCTC86450.023.10643640
ACATGTC93250.022.9331632
GAACTCC98300.022.17769221
TCTGAAC104150.020.93186618
CACATGT103200.020.6367931
CTGAACT107650.020.51697319
Produced by FastQC (version 0.11.2)